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rabbit antihuman cd3 antibody  (Agilent technologies)


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    Structured Review

    Agilent technologies rabbit antihuman cd3 antibody
    Rabbit Antihuman Cd3 Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+cd3/pm39111707-85-17-21
    Average 90 stars, based on 1 article reviews
    rabbit antihuman cd3 antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Vedolizumab-mediated integrin α4β7 blockade does not control HIV-1SF162 rebound after combination antiretroviral therapy interruption in humanized mice
    Article Snippet: .. Briefly, formalin-fixed, paraffin-embedded sections were stained with rabbit antihuman CD3 (DakoCytomation, Glostrup, Hovedstaden, Denmark), followed by staining with AF568 goat Copyright © 2019 Wolters Kluwe antirabbit IgG (Thermo Fisher Scientific). .. Confocal images were obtained by Carl Zeiss LSM 700 and analyzed with ZEN 2009 software (Carl Zeiss, Oberkochen, Ostalbkreis, Germany).

    Staining:

    Article Title: Vedolizumab-mediated integrin α4β7 blockade does not control HIV-1SF162 rebound after combination antiretroviral therapy interruption in humanized mice
    Article Snippet: .. Briefly, formalin-fixed, paraffin-embedded sections were stained with rabbit antihuman CD3 (DakoCytomation, Glostrup, Hovedstaden, Denmark), followed by staining with AF568 goat Copyright © 2019 Wolters Kluwe antirabbit IgG (Thermo Fisher Scientific). .. Confocal images were obtained by Carl Zeiss LSM 700 and analyzed with ZEN 2009 software (Carl Zeiss, Oberkochen, Ostalbkreis, Germany).

    Article Title: Adipose Tissue Hypertrophy, An Aberrant Biochemical Profile and Distinct Gene Expression in Lipedema.
    Article Snippet: Background: Lipedema is a common adipose tissue disorder affecting women, characterized by a symmetric subcutaneous adipose tissue deposition, particularly of the lower extremities.. Lipedema is usually underdiagnosed, thus remaining an undertreated disease.. Importantly, no histopathologic or molecular hallmarks exist to clearly diagnose the disease, which is often misinterpreted as obesity or lymphedema.

    Article Title: New evidence for a critical role of elastin in calcification of native heart valves: immunohistochemical and ultrastructural study with literature review.
    Article Snippet: Ida Perrotta, Emilio Russo, Caterina Camastra, Gemma Filice, Giulio Di Mizio, Federica Colosimo, Pietrantonio Ricci, Sandro Tripepi, Andrea Amorosi, Franco Triumbari & Giuseppe Donato Department of Ecology, University of Calabria, Rende, Department of Experimental and Clinical Medicine, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, and Department of Cardiology and Heart Surgery, S G Moscati Hospital, Avellino, Italy

    Article Title: Brain Invasion by CD4(+) T Cells Infected with a Transmitted/Founder HIV-1BJZS7 During Acute Stage in Humanized Mice.
    Article Snippet: Human immunodeficiency virus (HIV)-associated neurocognitive disorder (HAND) is one of the common causes of cognitive dysfunction andmorbidity among infected patients.. However, to date, it remains unknown if a transmitted/founder (T/F) HIV-1 leads to neurological disorders during acute phase of infection.. Since it is impossible to answer this question in humans, we studied NOD.Cg-Prkdc scid Il2rgtm1Wjl/SzJ mice (NSG) reconstituted with human PBMC (NSG-HuPBL), followed by the peritoneal challenge with the chronic HIV1JR-FL and the T/F HIV-1BJZS7, respectively.

    Incubation:

    Article Title: Ex vivo expansion of cord blood-CD34(+) cells using IGFBP2 and Angptl-5 impairs short-term lymphoid repopulation in vivo.
    Article Snippet: Cord blood-derived haematopoietic stem cells (CB-HSCs) are an attractive source for transplantation in haematopoietic disorders.. However, the yield of CB-HSCs per graft is limited and often insufficient, particularly for the treatment of adult patients.. Here we compare the capacity of three cytokine cocktails to expand CB-CD34 cells.

    Concentration Assay:

    Article Title: Ex vivo expansion of cord blood-CD34(+) cells using IGFBP2 and Angptl-5 impairs short-term lymphoid repopulation in vivo.
    Article Snippet: Cord blood-derived haematopoietic stem cells (CB-HSCs) are an attractive source for transplantation in haematopoietic disorders.. However, the yield of CB-HSCs per graft is limited and often insufficient, particularly for the treatment of adult patients.. Here we compare the capacity of three cytokine cocktails to expand CB-CD34 cells.

    Negative Control:

    Article Title: The Counter-Regulatory Effects of ESE-1 During Angiotensin II-Mediated Vascular Inflammation and Remodeling
    Article Snippet: .. The primary antibodies used in the study were as follows: rabbit antihuman ESE-1 (Orbigen, San Diego, CA), rabbit antihuman CD3, rabbit IgG fraction as negative control for CD3 (Dako, Carpinteria, CA), rat anti-mouse Mac-3, rat IgG 1 as negative control for Mac-3 (BD Pharmingen, San Jose, CA), rabbit antihuman vascular cell adhesion molecule (VCAM)-1 (Santa Cruz, Santa Cruz, CA), rabbit antihuman proliferating cell nuclear antigen (PCNA) (Santa Cruz), and rabbit IgG as negative control for rabbit Ab (Santa Cruz). .. Vectastain Elite ABC kits (Vector Labs, Burlingame, CA) were used for the immunohistochemical staining.

    other:

    Article Title: T-cell regulation of neutrophil infiltrate at the early stages of a murine colitis model.
    Article Snippet: Background: T-cells are a main target for antiinflammatory drugs in inflammatory bowel disease.. As the innate immune system is also implicated in the pathogenesis of these diseases, T-cell suppressors may not only inhibit T-cell-dependent production of proinflammatory mediators but also affect innate immune cell function.. Specifically, these drugs may impair innate immune cell recruitment and activation through inhibition of T-cells or act independent of T-cell modulation.

    Light Microscopy:

    Article Title: New evidence for a critical role of elastin in calcification of native heart valves: immunohistochemical and ultrastructural study with literature review.
    Article Snippet: Ida Perrotta, Emilio Russo, Caterina Camastra, Gemma Filice, Giulio Di Mizio, Federica Colosimo, Pietrantonio Ricci, Sandro Tripepi, Andrea Amorosi, Franco Triumbari & Giuseppe Donato Department of Ecology, University of Calabria, Rende, Department of Experimental and Clinical Medicine, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, and Department of Cardiology and Heart Surgery, S G Moscati Hospital, Avellino, Italy

    Immunohistochemical staining:

    Article Title: New evidence for a critical role of elastin in calcification of native heart valves: immunohistochemical and ultrastructural study with literature review.
    Article Snippet: Ida Perrotta, Emilio Russo, Caterina Camastra, Gemma Filice, Giulio Di Mizio, Federica Colosimo, Pietrantonio Ricci, Sandro Tripepi, Andrea Amorosi, Franco Triumbari & Giuseppe Donato Department of Ecology, University of Calabria, Rende, Department of Experimental and Clinical Medicine, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, Department of Pathology, School of Medicine, University Magna Graecia, Catanzaro, and Department of Cardiology and Heart Surgery, S G Moscati Hospital, Avellino, Italy

    Article Title: Brain Invasion by CD4(+) T Cells Infected with a Transmitted/Founder HIV-1BJZS7 During Acute Stage in Humanized Mice.
    Article Snippet: Human immunodeficiency virus (HIV)-associated neurocognitive disorder (HAND) is one of the common causes of cognitive dysfunction andmorbidity among infected patients.. However, to date, it remains unknown if a transmitted/founder (T/F) HIV-1 leads to neurological disorders during acute phase of infection.. Since it is impossible to answer this question in humans, we studied NOD.Cg-Prkdc scid Il2rgtm1Wjl/SzJ mice (NSG) reconstituted with human PBMC (NSG-HuPBL), followed by the peritoneal challenge with the chronic HIV1JR-FL and the T/F HIV-1BJZS7, respectively.

    Immunopeptidomics:

    Article Title: Histological, histochemical and immunohistochemical study of the lymph nodes of the one humped camel (Camelus dromedarius).
    Article Snippet: Prescapular, femoral, mesenteric, mediastinal and splenic lymph nodes from nine camels of one to 12 years of age were studied.. There were no obvious structural differences between these different lymph nodes or between the ages.. The lymph nodes were surrounded by a capsule formed of two layers, an outer thicker layer of connective tissue and an inner thinner layer mainly of smooth muscles.



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    Immunofluorescent micrographs showing colocalization of CD20-positive B lymphocytes and <t>CD3-positive</t> T lymphocytes in the control ( A – C ) and inflamed lacrimal sac 1 month ( D – F ) and 3 months ( G – I ) after Staphylococcus aureus inoculation. In control tissue, a small number of CD20-positive ( A , red) and CD3-positive lymphocytes ( B , green) were codistributed ( C ) in the lamina propria. One month after inoculation, numerous CD20-positive ( D , red) and CD3-positive lymphocytes ( E , green) were coinfiltrated ( F ) into the lamina propria. Three months after inoculation, the numbers of CD20-positive ( G , red) and CD3-positive ( H , green) lymphocytes codistributed ( I ) were decreased compared with 1 month. Notes: Bars = 65 μm; blue, diamidino-2-phenylindole-stained nuclei.
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    Cell Marque rabbit monoclonal antihuman cd3 mrq-39
    Representative hematoxylin-stained tissue sections showing <t>CD3</t> + and CD8 + T cells and CD20 + B cells in stage III human colon cancers. The core tumor (CT) and the invasive margin (IM) are shown with the demarcation of the IM ( red line ; see
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    Image Search Results


    Immunofluorescent micrographs showing colocalization of CD20-positive B lymphocytes and CD3-positive T lymphocytes in the control ( A – C ) and inflamed lacrimal sac 1 month ( D – F ) and 3 months ( G – I ) after Staphylococcus aureus inoculation. In control tissue, a small number of CD20-positive ( A , red) and CD3-positive lymphocytes ( B , green) were codistributed ( C ) in the lamina propria. One month after inoculation, numerous CD20-positive ( D , red) and CD3-positive lymphocytes ( E , green) were coinfiltrated ( F ) into the lamina propria. Three months after inoculation, the numbers of CD20-positive ( G , red) and CD3-positive ( H , green) lymphocytes codistributed ( I ) were decreased compared with 1 month. Notes: Bars = 65 μm; blue, diamidino-2-phenylindole-stained nuclei.

    Journal: Clinical Ophthalmology (Auckland, N.Z.)

    Article Title: Structural changes in the lacrimal sac epithelium and associated lymphoid tissue during experimental dacryocystitis

    doi: 10.2147/OPTH.S26048

    Figure Lengend Snippet: Immunofluorescent micrographs showing colocalization of CD20-positive B lymphocytes and CD3-positive T lymphocytes in the control ( A – C ) and inflamed lacrimal sac 1 month ( D – F ) and 3 months ( G – I ) after Staphylococcus aureus inoculation. In control tissue, a small number of CD20-positive ( A , red) and CD3-positive lymphocytes ( B , green) were codistributed ( C ) in the lamina propria. One month after inoculation, numerous CD20-positive ( D , red) and CD3-positive lymphocytes ( E , green) were coinfiltrated ( F ) into the lamina propria. Three months after inoculation, the numbers of CD20-positive ( G , red) and CD3-positive ( H , green) lymphocytes codistributed ( I ) were decreased compared with 1 month. Notes: Bars = 65 μm; blue, diamidino-2-phenylindole-stained nuclei.

    Article Snippet: The same sections were also incubated with rabbit antihuman CD3 antibody (1:100; Sigma-Aldrich, St Louis, MO) to colocalize T lymphocytes.

    Techniques: Staining

    Reagents for IHC Staining.

    Journal: Toxicologic Pathology

    Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

    doi: 10.1177/0192623320970534

    Figure Lengend Snippet: Reagents for IHC Staining.

    Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

    Techniques: Staining, Concentration Assay, Incubation

    The staining intensity of a particular IHC method is influenced more by the biomarker than the choice of stainer. Data are shown as the CV (calculated as the SD divided by the mean) for the average OD within each staining run for each biomarker on a given instrument for all tissues. The box plots represent the distribution of the data, where the box defines the range encompassing values between the 25th and 75th quartiles, the line within the box is drawn at the median OD, and the whiskers demonstrate the expected variation in the data. Dots located over some boxes (eg, CD3 on instrument 0083) plot data that fell beyond the whiskers, which are indicative of staining runs with higher than expected variability. For Ki-67, the expanded ranges (longer boxes) on all 3 stainers denote that staining was more variable across runs for sections containing lymphoid tissue (see ). Instrument models: AutostainerPlus Link, Nos. 0010 and 0083; Autostainer Link 48, No. 0151. CV indicates coefficient of variation; DAB, 3,3′-diaminobenzidene; IHC, immunohistochemical; OD, optical density; SD, standard deviation.

    Journal: Toxicologic Pathology

    Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

    doi: 10.1177/0192623320970534

    Figure Lengend Snippet: The staining intensity of a particular IHC method is influenced more by the biomarker than the choice of stainer. Data are shown as the CV (calculated as the SD divided by the mean) for the average OD within each staining run for each biomarker on a given instrument for all tissues. The box plots represent the distribution of the data, where the box defines the range encompassing values between the 25th and 75th quartiles, the line within the box is drawn at the median OD, and the whiskers demonstrate the expected variation in the data. Dots located over some boxes (eg, CD3 on instrument 0083) plot data that fell beyond the whiskers, which are indicative of staining runs with higher than expected variability. For Ki-67, the expanded ranges (longer boxes) on all 3 stainers denote that staining was more variable across runs for sections containing lymphoid tissue (see ). Instrument models: AutostainerPlus Link, Nos. 0010 and 0083; Autostainer Link 48, No. 0151. CV indicates coefficient of variation; DAB, 3,3′-diaminobenzidene; IHC, immunohistochemical; OD, optical density; SD, standard deviation.

    Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

    Techniques: Staining, Biomarker Assay, Immunohistochemical staining, Standard Deviation

    Staining intensity may be impacted unexpectedly by inadvertent adjustments to the IHC protocol design. In this example, Ki-67-positive cells in 4-µm-thick step sections exhibit stronger staining when processed using a protocol in which the incubation in primary antibody is followed rapidly by application of the visualization reagent (ie, the standard protocol [column A]) relative to 4-µm thick step sections in which the primary antibody was followed by a 35-min incubation in buffer before addition of the visualization reagent (column B); the extended buffer incubation for Ki-67 corresponded to the time needed to accommodate another biomarker in the same staining run (CD45, which requires incubation with a secondary antibody prior to application of the visualization reagent). The decreased staining intensity associated with extended rinsing for some staining runs likely explains the higher variation in Ki-67 labeling across instruments (see ) relative to labeling provided by the other biomarkers tested in this study (CD3, CD45, F4/80). IHC indicates immunohistochemical.

    Journal: Toxicologic Pathology

    Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

    doi: 10.1177/0192623320970534

    Figure Lengend Snippet: Staining intensity may be impacted unexpectedly by inadvertent adjustments to the IHC protocol design. In this example, Ki-67-positive cells in 4-µm-thick step sections exhibit stronger staining when processed using a protocol in which the incubation in primary antibody is followed rapidly by application of the visualization reagent (ie, the standard protocol [column A]) relative to 4-µm thick step sections in which the primary antibody was followed by a 35-min incubation in buffer before addition of the visualization reagent (column B); the extended buffer incubation for Ki-67 corresponded to the time needed to accommodate another biomarker in the same staining run (CD45, which requires incubation with a secondary antibody prior to application of the visualization reagent). The decreased staining intensity associated with extended rinsing for some staining runs likely explains the higher variation in Ki-67 labeling across instruments (see ) relative to labeling provided by the other biomarkers tested in this study (CD3, CD45, F4/80). IHC indicates immunohistochemical.

    Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

    Techniques: Staining, Incubation, Biomarker Assay, Labeling, Immunohistochemical staining

    Representative hematoxylin-stained tissue sections showing CD3 + and CD8 + T cells and CD20 + B cells in stage III human colon cancers. The core tumor (CT) and the invasive margin (IM) are shown with the demarcation of the IM ( red line ; see

    Journal: JNCI Cancer Spectrum

    Article Title: Contribution of Immunoscore and Molecular Features to Survival Prediction in Stage III Colon Cancer

    doi: 10.1093/jncics/pkaa023

    Figure Lengend Snippet: Representative hematoxylin-stained tissue sections showing CD3 + and CD8 + T cells and CD20 + B cells in stage III human colon cancers. The core tumor (CT) and the invasive margin (IM) are shown with the demarcation of the IM ( red line ; see "Methods") separating malignant glands/cells from peritumoral stroma.

    Article Snippet: Sections were incubated at 37°C with primary antibodies: rabbit monoclonal antihuman CD3 (MRQ-39, Cell Marque, Rocklin, CA), mouse antihuman monoclonal antibodies against CD8 + (C8/144B, Cell Marque), and CD20 + (MO755, clone L26, DAKO).

    Techniques: Staining

    Univariate associations of clinical, immune, and molecular features (in best functional form) with DFS

    Journal: JNCI Cancer Spectrum

    Article Title: Contribution of Immunoscore and Molecular Features to Survival Prediction in Stage III Colon Cancer

    doi: 10.1093/jncics/pkaa023

    Figure Lengend Snippet: Univariate associations of clinical, immune, and molecular features (in best functional form) with DFS

    Article Snippet: Sections were incubated at 37°C with primary antibodies: rabbit monoclonal antihuman CD3 (MRQ-39, Cell Marque, Rocklin, CA), mouse antihuman monoclonal antibodies against CD8 + (C8/144B, Cell Marque), and CD20 + (MO755, clone L26, DAKO).

    Techniques: Functional Assay